Computational protocol: Hepatitis C Virus-Induced Cytoplasmic Organelles Use the Nuclear Transport Machinery to Establish an Environment Conducive to Virus Replication

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Protocol publication

[…] Immunofluorescence and western blotting were done as previously described . Further details and a list of primary and secondary antibodies are provided in the extended . Quantification of nuclear and cytoplasmic fluorescence levels was done using ImageJ. Cell outlines were determined using DIC images. The percent cytoplasmic colocalization of Nups with HCV core or the NS5A protein was calculated in ImageJ using the Manders overlap coefficients as previously described . Pearson's colocalization coefficients were calculated as previously described using the JACoP plugin for ImageJ . […]

Pipeline specifications

Software tools ImageJ, JACoP
Application Microscopic phenotype analysis
Organisms Classical swine fever virus
Diseases Hepatitis C