Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 2
Release date: Dec 2 2009
Last update date: Mar 21 2012
Access: Public
Diseases: Meningitis
Dataset link Microarray Analysis of Temperature-induced Transcriptome of Streptococcus suis serotype 2

Experimental Protocol


A cDNA microarray imprinted with 2156 genes representing about 98% of Streptococcus suis serotype 2 genome was used for transcriptome analysis . For two-sample (reference vs. test) microarray hybridization, four independent bacterial cultures from each condition were prepared as biological replicates for RNA isolation . Accordingly, for each time point, four dual-fluorescence-labeled cDNA probes were prepared to hybridize with four slides, respectively. Pairwise comparisons were made using dye swaps to avoid labeling bias. A ratio of mRNA levels (test/reference) was calculated for each gene. Significant changes of gene expression were identified with the SAM software . After the SAM analysis, only genes with at least 2-fold changes in expression were collected for further analysis.

Repositories


GEO

GSE19215

BioProject

PRJNA120833

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Contact


Ruifu Yang