|Application:||Gene expression microarray analysis|
|Number of samples:||2|
|Release date:||Dec 2 2009|
|Last update date:||Mar 21 2012|
|Dataset link||Microarray Analysis of Temperature-induced Transcriptome of Streptococcus suis serotype 2|
A cDNA microarray imprinted with 2156 genes representing about 98% of Streptococcus suis serotype 2 genome was used for transcriptome analysis . For two-sample (reference vs. test) microarray hybridization, four independent bacterial cultures from each condition were prepared as biological replicates for RNA isolation . Accordingly, for each time point, four dual-fluorescence-labeled cDNA probes were prepared to hybridize with four slides, respectively. Pairwise comparisons were made using dye swaps to avoid labeling bias. A ratio of mRNA levels (test/reference) was calculated for each gene. Significant changes of gene expression were identified with the SAM software . After the SAM analysis, only genes with at least 2-fold changes in expression were collected for further analysis.