Computational protocol: Peri-implant crevicular fluid levels of cathepsin-K, RANKL, and OPG around standard, short, and mini dental implants after prosthodontic loading

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Protocol publication

[…] After the osseointegration period was completed, PICF sampling and clinical measurements were done prior to installation and prosthetic loading was applied in the same day. Sampling and measurements, followed by the installation, were repeated on post-loading days 2, 14 and 90. Panoramic radiographs were taken after surgical procedures and at 90 days.The plaque index modified by Mombelli et al. (mPI) [] and gingival index modified by Loe and Silness (mGI) [] were used. The mPI and mGI were recorded around each implant at four locations: mesiobuccally, distobuccally, mesiolingually, and distolingually. The average of the mPI and mGI scores at the four locations for each implant were calculated.Using a calibrated pressure-sensitive plastic periodontal probe (the Colorvue™, Hu-Friedy, US), the distance between the marginal border of the gingiva and the tip of the probe was measured and considered to be the probing pocket depth (PPD). The PPD was recorded for six locations around each implant: mesiobuccally, bucally, distobuccally, mesiolingually, lingually and distolingually []. The average of the six PPD scores collected for each implant was calculated. The bleeding tendency of the peri-implant mucosa, as a measure of soft-tissue inflammation, was evaluated using the modified sulcular bleeding index (mSBI) [], measured at four sites around the implant (mesially, distally, and at the buccal and lingual mid-point).The width of peri-implant keratinized mucosa (KT) was measured in millimeters on the facial aspect (mesially, at the mid-point and distally). Differences in color, texture, and mobility between the keratinized mucosa and the lining mucosa served as markers for the detection of the muco-gingival junction. KT was then measured as the distance between the gingival margin and the muco-gingival junction, and measurements were rounded to the nearest millimeter []. The average of the values collected for a given implant was then determined.Radiographs were taken at each of the clinical procedure appointments at implant placement and 90 days after prosthetic loading. Measurements on panoramic radiographs obtained were carried out by measuring the image files after transfer to the image processing program (ImageJ 1.46r, NIH, Maryland, USA), and the actual amount of bone loss (MBLR) was determined. Vertical measurements of the bone level adjacent to the implants were made from the top of the implant, which provides a fixed reference point. Calibration of the measured increments of bone change is necessary in determining actual bone loss from radiographic measurements, particularly on panoramic radiographs, which generally provide an enlarged image of teeth and implants. Formulas from Manz [] were used for calibration. These calibrated (i.e. actual) measurements from baseline and follow-up appointments were compared for a given implant to determine vertical bone height changes. [...] All data were first analyzed descriptively and were presented as mean±SD values. We used the Wilcoxon signed rank test to compare the baseline data with the data from day 2, 14 and 90 post-loading for each group and the Friedman test to compare parameters among the three implant groups at baseline and at each post-loading follow-up day. The significance level was set at p<0.05. Statistical analysis was performed with SPSS Statistics for Windows version 15.0 (SPSS, Inc., Chicago, IL, USA). […]

Pipeline specifications

Software tools ImageJ, SPSS
Applications Miscellaneous, Microscopic phenotype analysis
Diseases Gingival Pocket, Tooth Abnormalities, Alveolar Bone Loss