Computational protocol: Arginase activity in pathogenic and non-pathogenic species of Leishmania parasites

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Protocol publication

[…] All nucleotide sequences are deposited in the GenBank database under the accession numbers KU641750 (L. major MRHO/IR/75/ER), KU641752 (L. tarentolae Tar II ATCC 30267) and KU641753 (L. tropica MOHM/IR/09/Khamesipour-Mashhad). The analysis were carried out using the BLAST analysis for amino acid and nucleic acid pairwise sequence alignment [] and ClustalW programs for multiple sequence alignments [] as implemented in BioEdit software, version 7.2.5 [] and improved in MAFFT []. Finally all sequences were aligned and a phylogenetic tree was constructed by MEGA v5.05 software []. […]

Pipeline specifications

Software tools Clustal W, BioEdit, MAFFT, MEGA-V
Applications Phylogenetics, GWAS
Organisms Mus musculus, Homo sapiens, Cyclophiops major, Leishmania tarentolae, Leishmania major strain Friedlin
Diseases Infection
Chemicals Amino Acids, Arginine, Polyamines