Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 2
Release date: Apr 16 2015
Last update date: Oct 17 2018
Access: Public
Dataset link Identification of RNAs associated with telomeres by enChIP-RNA-Seq

Experimental Protocol


RNAs associated with telomeres were identified by using the enChIP technology combined with deep sequencing using Illumina Miseq. Briefly, to isolate telomeres, a TAL protein, Telomere-TAL (Tel-TAL), recognizing a 19-bp sequence containing an array of TTAGGG (telomere repeats) was fused with 3xFLAG tag and NLS (3xFN-Tel-TAL) and LexA protein (3xFNLDD)11 were expressed in a mouse hematopoietic cell line, Ba/F3, respectively. The cells were crosslinked with formaldehyde, and crosslinked chromatin was fragmented by sonication. Subsequently, chromatin complexes containing 3xFN-Tel-TAL or 3xFNLDD were immunoprepicitated with anti-FLAG M2 Ab. Supplementary URL: http://www.nature.com/srep/2013/131108/srep03171/full/srep03171.html

Repositories


GEO

GSE60425

ArrayExpress

E-GEOD-60425

ENA

SRP045319

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Contact


Daisuke Okuzaki

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