Computational protocol: RNA editing in RHOQ promotes invasion potential in colorectal cancer

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Protocol publication

[…] Crystals of edited RhoQ proteins were grown under previously reported conditions (20% PEG3350, 200 mM NaCl, and 0.1 M MES, pH 6.0) using hanging drop vapor diffusion. Crystals from the drop were transferred to reservoir solutions supplemented with 20% glycerol and mounted under a cryostream at Pohang Accelerator Laboratory (7A). Data were processed according to a previously published method (). Then, using a previously reported structure (2ATX), REFMAC5 () generated the electron-density map. Finally, the edited structure was analyzed using a crystallographic object-oriented toolkit (COOT; ). […]

Pipeline specifications

Software tools REFMAC5, Coot
Application Protein structure analysis
Organisms Homo sapiens
Diseases Neoplasms, Colorectal Neoplasms