|Application:||Gene expression microarray analysis|
|Number of samples:||10|
|Release date:||Dec 31 2014|
|Last update date:||Aug 13 2018|
|Diseases:||Graft vs Host Disease|
|Dataset link||ATAXIN2 is crucial for megakaryocyte protein homeostasis and proper platelet functionality|
The human megakaryoblastic cell line Meg01 was transduced with either a short hairpin against ATXN2 (sh95) or a short hairpin control (shc002). 48 hours after transduction, cells were sorted for successful transduction by GFP-expression, lysed and subsequently loaded on a sucrose gradient to separate mRNA based on the amount of ribosomes attached to it. The first eight fractions of the gradient containing subpolysomal, untranslated mRNA were pooled as were the remaining eight fractions containing polyribosomal, translated mRNA. Subpolysomal and polysomal mRNA from three independent experiments were then hybridized to an Illumina beadchip array.