Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 4
Release date: Jan 15 2013
Last update date: Apr 7 2014
Access: Public
Taxon: Bacillus subtilis
Dataset link Bacillus subtilis Rnase Y activity in vivo analysed by tiling microarrays

Experimental Protocol


A four arrays study of the transcriptome profiles of the B. subtilis RNase Y depletion strain SSB447 (Shahbabian et al., 2009) in which the gene rny is under the control of an IPTG inducible promoter. Rnase Y depleted cells (IPTG-) are compared to the transcript profiles of non depleted cells (IPTG+). As the rny gene is essential, depletion can only be partial. Here we carefully selected the time point for taking the sample in order to minimize growth rate effects and to obtain an highly reproducible data set.

Repositories


GEO

GSE38897

ArrayExpress

E-GEOD-38897

BioProject

PRJNA169144

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Contact


Harald Putzer

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