Computational protocol: Expression profile of the Plasmodium falciparum intra-erythrocytic stage protein, PF3D7_1363700

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Protocol publication

[…] Initial efforts to identify novel sporozoite surface or secreted proteins that may be involved in sporozoite invasion of host tissues were performed by data mining PlasmoDB []. The data-mining search specifically aimed to select for uncharacterized genes that expressed protein during the sporozoite stage, although future analyses suggested otherwise, and was predicted to be a surface or secreted protein with a signal peptide. The PF3D7_1363700 protein was identified and protein sequences were subjected to BLAST analysis within PlasmoDB [] and National Center for Biotechnology Information (NCBI) [] databases to determine protein similarity. Additional analyses were obtained using Expert Protein Analysis System (ExPASy) proteomic tools such as TargetP and SignalP for signal peptide/anchor predictions, PSORTII, TMAP, and DGPI for localization, transmembrane and GPI anchor predictions, and Pfam and Prosite for classifying family proteins and identifying functional sites, and other patterns/profiles []. […]

Pipeline specifications

Software tools TargetP, SignalP
Databases PlasmoDB ExPASy
Application Protein sequence analysis
Organisms Plasmodium falciparum
Diseases Malaria, Malaria, Falciparum