Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 14
Release date: Feb 1 2012
Last update date: Jul 26 2018
Access: Public
Diseases: Pancreatic Neoplasms, Cervical Intraepithelial Neoplasia
Dataset link The chromatin protein Nupr1 regulates RelB-dependent NF-kB events necessary for pancreatic cancer development

Experimental Protocol


Since Nupr1 belongs to the HMG family of chromatin remodelers with transcriptional co-factor activity, Nupr1 could increase cell survival in a nutrient-deprived microenvironment by activating the expression of pro-survival genes. Moreover, considering that RelB, and not RelA/p65, is essential to the Nupr1-mediated survival mechanism that takes place upon nutrient deprivation-induced stress, we made the hypothesis that the two NF-kB transcription factors should activate different sets of genes among which a pivotal pro-survival gene would be exclusively dependent on RelB. In order to test this two hypothesis, an Affymetrix microarray analysis was performed using pancreatic cancer cells transfected with siCtrl or siNupr1 and cultured for 3, 6 or 9 hrs in EBSS; or transfected with siRelB, siRelA/p65 or siCtrl and cultured for 9 hrs in EBSS or Mock.

Repositories


GEO

GSE35463

ArrayExpress

E-GEOD-35463

BioProject

PRJNA152337

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Contact


Ezequiel Calvo
Ezequiel L Calvo