Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 24
Release date: Jan 1 2016
Last update date: Aug 13 2018
Access: Public
Diseases: Hodgkin Disease, Neoplasms
Chemicals: Oxygen
Dataset link Gene expression profiling of L-540, SUP-HD1, KM-H2 and L-428 Hodgkin lymphoma cell lines after in vitro and in vivo treatment with the dual PI3K/ERK inhibitor AEZS-136 [2 hours]

Experimental Protocol


The Hodgkin lymphoma cell lines were obtained from the DSMZ (Braunschweig, Germany, EU). Cells were routinely maintained in RPMI medium 1640 (Lonza, Basel, Switzerland) supplemented with 20% FBS (Lonza) and 2 mM glutamine (Lonza). Cells were maintained at 37°C in a water-saturated atmosphere of 5% CO2 in air. 4x106 HL cells were seeded in 75 cm2 flask and, after 24 hrs, cells were treated with 10 µM AEZS-136 (Aeterna Zentaris, Frankfurt, Germany, EU) in culture medium for 2 hours. At the end of treatment, cells were collected and RNA extracted.

Repositories


GEO

GSE71150

ArrayExpress

E-GEOD-71150

BioProject

PRJNA290466

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Contact


Giuliano Stirparo
Giuliano Giuseppe Stirparo