Computational protocol: Wheat Brassinosteroid-Insensitive1 (TaBRI1) Interacts with Members of TaSERK Gene Family and Cause Early Flowering and Seed Yield Enhancement in Arabidopsis

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Protocol publication

[…] The full length cDNA sequence was used to search homologous sequences and percentage sequence similarity via BLASTX and BLASTP in NCBI database (http://www.ncbi.nlm.nih.gov). For domain analysis TaBRI1 protein sequence was analyzed by CDD (Conserved domain database) and SMART (Simple Modular Architecture Research Tool). Multiple sequence alignment was carried out by CLUSTALW program and phylogenetic tree was constructed using MEGA6 program by neighbor-joining method. […]

Pipeline specifications

Software tools BLASTX, BLASTP, Clustal W, MEGA
Databases SMART CDD Full Length cDNA
Application Phylogenetics
Organisms Arabidopsis thaliana, Triticum aestivum, Bovine orthopneumovirus
Diseases Drug Hypersensitivity