Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 9
Release date: Jun 27 2018
Last update date: Nov 28 2018
Access: Public
Chemicals: Calcium
Dataset link Stable STIM1 knockdown in human neural precursors derived from hESC line, H9

Experimental Protocol


STIM1 knockdown in human embryonis stem cell derived neural progenitor cells (NPCs) by using ShERWOOD-UltramiR short hairpin RNA (shRNA). Hence we designed our RNA seq in such a way that we can compare the differential gene expression data using STIM1 Knockdown (KD) as test group, shRNA non-targetted control (NTC) and wild type NPC (WT) as control groups. Biological triplicates were performed for each sample consisting of RNA isolated from wild type NPCs.

Repositories


GEO

GSE109111

ENA

SRP128928

BioProject

PRJNA429650

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Contact


Gaiti Hasan