Computational protocol: The multivesicular body is the major internal site of prion conversion

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Protocol publication

[…] Confocal images were obtained with the Zeiss LSM 510 or the Zeiss LSM 780 microscope using a 63×, 1.4 NA objective. The identical confocal settings were used when comparing the relative fluorescence intensity between images. Super-resolution images were obtained with the Delta Vision Omx microscope (GE Health Care) using a 60× 1.42 NA objective. The super-resolution data were processed using ImageJ software. The MetaMorph colocalization application (Molecular Devices) was used to quantify colocalization between proteins in individual cells. […]

Pipeline specifications

Software tools ImageJ, MetaMorph
Application Microscopic phenotype analysis