Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 18
Release date: Sep 10 2018
Last update date: Nov 28 2018
Access: Public
Dataset link ER proteostasis and temperature differentially impact the mutational tolerance of influenza hemagglutinin

Experimental Protocol


RNA-seq characterizing a clonal HEK293T-Rex cell line, expressing DHFR ATF6f, Tet XBP1s, and the tetracycline repressor. These cell lines were treated with small molecules for 24 hours (in triplicate) to modulate the proteostasis environment in a stress-independent manner, at either 37C or 39C. XBP1s was activated by treatment with 0.1 ug/mL Doxycycline; ATF6f/XBP1s were activated by treatment with 0.1 ug/mL Doxycycline and 1 uM TMP; basal cells were vehicle-treated (0.01% DMSO). These cells were previously characterized in Shoulders et al. Cell Reports, 2013.

Repositories


GEO

GSE115168

ENA

SRP149449

BioProject

PRJNA473993

Download


Contact


Matthew Shoulders