Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 35
Release date: Mar 24 2015
Last update date: Apr 5 2017
Access: Public
Taxon: Homo sapiens
Dataset link Gene expression profiles of fibroblasts and induced pluripotent stem cells (iPSCs) from individuals with Zellweger spectrum disorder (ZSD), a class of peroxisome biogenesis disorder, and healthy controls

Experimental Protocol


Dermal fibroblast cultures from 6 PBD-ZSD patient and 3 healthy control donors were reprogrammed into iPSCs by transfection with retroviruses designed to express the human OCT4, SOX2, KLF4 and c-MYC genes. Fibroblasts and iPSCs were cultured in 1:1 ratio of DMEM:F12 medium supplemented with 20% KSR at 37°C with 5% CO2 until confluence for RNA extraction. The overall goal was to identify genes that are differentially expressed between PBD-ZSD patient and healthy control cells in order to gain insights into the pathomechanisms of disease.

Repositories


GEO

GSE43996

BioProject

PRJNA188315

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Contact


Joseph Hacia