Computational protocol: An unstructured 5′-coding region of the prfA mRNA is required for efficient translation

Similar protocols

Protocol publication

[…] RNA sequences of different constructs/mRNAs were analysed using the RNAfold web server of the Vienna RNA package (http://rna.tbi.univie.ac.at/egi-bin/RNAfold.cgi). For each sequence, the minimum free energy in kcal mol−1 was predicted (). […]

Pipeline specifications

Software tools RNAfold, ViennaRNA
Application RNA structure analysis
Organisms Listeria monocytogenes, Homo sapiens, Escherichia coli