|Application:||Gene expression microarray analysis|
|Number of samples:||6|
|Release date:||Jul 24 2017|
|Last update date:||Aug 23 2018|
|Dataset link||Expression data from the control and Jmjd1c KD ESCs|
J1 ESCs were infected with lentiviral supernatants of shNT-pLKO.1, shJmjd1c#3-pLKO.1 or shJmjd1c#4-pLKO.1. One day post-infection, ESCs were selected with puromycin at the final concentration of 1.5ug/ml for 2 days. Subsequently, the puromycin-resistent ESCs were re-plated onto dishes, cultured for 4 days, and collected for RNA isolation. In total, six RNA samples were prepared from the two independent biological duplicates. Microarray analysis was performed with the Affymetrix GeneChip® Mouse Genome 430 2.0 Arrays. The procedures including RNA extraction, cDNA synthesis, labeling, hybridization, washing, and scanning were carried out according to the standard Affymetrix protocol by the Shanghai Biotechnology Corporation.