Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Jul 24 2017
Last update date: Aug 23 2018
Access: Public
Dataset link Expression data from the control and Jmjd1c KD ESCs

Experimental Protocol


J1 ESCs were infected with lentiviral supernatants of shNT-pLKO.1, shJmjd1c#3-pLKO.1 or shJmjd1c#4-pLKO.1. One day post-infection, ESCs were selected with puromycin at the final concentration of 1.5ug/ml for 2 days. Subsequently, the puromycin-resistent ESCs were re-plated onto dishes, cultured for 4 days, and collected for RNA isolation. In total, six RNA samples were prepared from the two independent biological duplicates. Microarray analysis was performed with the Affymetrix GeneChip® Mouse Genome 430 2.0 Arrays. The procedures including RNA extraction, cDNA synthesis, labeling, hybridization, washing, and scanning were carried out according to the standard Affymetrix protocol by the Shanghai Biotechnology Corporation.

Repositories


GEO

GSE89709

BioProject

PRJNA352984

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Contact


Ying Jin

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