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16S ribosomal RNA reconstruction software tools | Shotgun metagenomic sequencing data analysis

16S ribosomal RNA reconstruction software tools | Shotgun metagenomic sequencing data analysis Metagenomic data, which contains sequenced DNA reads of uncultured microbial species from environmental samples, provide a unique opportunity to thoroughly analyze microbial species that have never been identified before. Reconstructing 16S ribosomal RNA, a phylogenetic marker gene, is usually required to analyze the composition of the metagenomic data. However, massive volume of dataset, high sequence similarity between related species, skewed microbial abundance and lack of reference genes make 16S rRNA reconstruction difficult.
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