|Application:||Gene expression microarray analysis|
|Number of samples:||4|
|Release date:||Mar 26 2018|
|Last update date:||Sep 6 2018|
|Dataset link||microRNA-218 modulates gene expression in fibroblast-like synovial cells in rheumatoid arthritis|
Number of sample:4 sample Method: For a transient transfection approach with the aim to inhibit or enhance the miR-218 function, RA-FLSs were transfected with synthetic precursor miRNA (pre-miR), with inhibitors of miR-218 (anti-miR), or with scrambled controls (Pre-miR/Anti-miR Negative Control #1; Ambion/Applied Biosystems, Foster City, CA) at a final concentration of 100 nM with the use of Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA). After transfection we extracted RNA using mirVana miRNA Isolation kit(Ambion/Applied Biosystems). We investigated the gene expression after transfection of pre/anti-miR-218 using microarray.