Computational protocol: Protonophore properties of hyperforin are essential for its pharmacological activity

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Protocol publication

[…] Initial analysis of electrophysiological and photomultiplier-based pH imaging experiments were performed with FitMaster (HEKA), and the camera-based FFN511, SBFI and pH imaging experiments were analyzed using TILLvisION (TILL Photonics). IGOR Pro (Wave Metrics, Lake Oswego, OR, USA), ImageJ (NIH, USA) and Adobe Illustrator were used for further analysis and for preparing the figures. Where applicable the data was averaged and given as means ± S.E.M for an indicated number of cells. Two tailed two-sample equal variance Student's t-tests (Microsoft Excel) were used to test for significant differences. The theoretical reversal potentials for proton currents were calculated via the Nernst equation with R = 8.314 J/(mol*K), T = 297 K, F = 96485 C/mol, z = 1, and [H+]ext and [H+]int representing the external and internal proton concentrations, respectively. […]

Pipeline specifications

Software tools ImageJ, Adobe Illustrator
Applications Miscellaneous, Microscopic phenotype analysis
Chemicals Sodium