|Application:||Gene expression microarray analysis|
|Number of samples:||12|
|Release date:||Nov 15 2012|
|Last update date:||Nov 16 2012|
|Dataset link||Heterologous hybridization to an oligonucleotide microarray monitors differential gene expression in Pinus radiata exposed to long-term Ethephon exposure|
Series of 2-color, 2 condition experiments in 12 180k arrays. Main comparison is within tissues exposed to 0 [control] or 1 mg Ethephon. 2nd level of comparison is between tissues [bark, xylem scraping, xylem]. Third level of comparison is between time [ 1 or 8 week exposure]. One slide is hybridized with cRNA generated from control and treated tissues with the same duration of exposure to Ethephon. Two biological replicates [each biological rep is a 2 y old cutting propagated clone] for treated plants whilst control consists of RNA pooled, in equal proportions [estimated by UV absorbance], from 2 untreated biological replicates.]. Dyes used for each sample are indicated in sample description.
Joseph Gogo Dubouzet