Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Nov 15 2012
Last update date: Nov 16 2012
Access: Public
Chemicals: Flavonoids, Lanolin
Dataset link Heterologous hybridization to an oligonucleotide microarray monitors differential gene expression in Pinus radiata exposed to long-term Ethephon exposure

Experimental Protocol


Series of 2-color, 2 condition experiments in 12 180k arrays. Main comparison is within tissues exposed to 0 [control] or 1 mg Ethephon. 2nd level of comparison is between tissues [bark, xylem scraping, xylem]. Third level of comparison is between time [ 1 or 8 week exposure]. One slide is hybridized with cRNA generated from control and treated tissues with the same duration of exposure to Ethephon. Two biological replicates [each biological rep is a 2 y old cutting propagated clone] for treated plants whilst control consists of RNA pooled, in equal proportions [estimated by UV absorbance], from 2 untreated biological replicates.]. Dyes used for each sample are indicated in sample description.

Repositories


GEO

GSE42300

ArrayExpress

E-GEOD-42300

BioProject

PRJNA180987

Download


Contact


Joseph Dubouzet
Joseph Gogo Dubouzet