Dataset features

Specifications


Application: ChIP-seq analysis
Number of samples: 12
Release date: Jan 5 2011
Last update date: Sep 28 2018
Access: Public
Diseases: Breast Neoplasms, Neoplasms, Uterine Neoplasms
Chemicals: Estradiol, Estrogens
Genes: ESR1
Computational protocol: MDscan, ROCR, MACS, CENTDIST, TRANSFAC
Dataset link Tissue-type specific estrogen signaling in breast and uterine cancer cells

Experimental Protocol


In order to explore common and distinctive features of ERα (estrogen receptor alpha) binding profiles between breast and uterus, we generated eight ChIP-Seq libraries for the four cell lines (MCF7, T47D, ECC1 and Ishikawa) under two different treatments (E2, ethanol). In addition, we generated four control libraries for the four cell lines. For all treatment libraries, we generated about 7-12 million unique tags each. ER antibody catalog number is (Santa Cruz,sc-543).

Repositories


GEO

GSE23893

ArrayExpress

E-GEOD-23893

ENA

SRP003480

BioProject

PRJNA130441

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Contact


Cheng Wei Chang

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Dataset publications

 (2)