Computational protocol: Expression and purification of truncated diphtheria toxin, DT386, in Escherichia coli: An attempt for production of a new vaccine against diphtheria

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Protocol publication

[…] B-cell epitope prediction (BCPred) (10) and EpiJen (11) web-based servers have been used to predict the linear B-cell and T-cell epitopes of DT386 protein (GenBank: BAL14546.1). In order to predict the B-cell epitopes, BCpreds: B-cell epitope prediction server was used; first, the primary sequence (amino acid sequence in plain format) was added to the chart and BCpred was selected for fixed length epitope prediction. Furthermore, the epitope length was selected as 20 residues and 75% as specificity. On the other hand, EpiJen v1.0 server was used to predict the T-cell epitopes. In order to use this server, the protein sequence was inserted in plain format. Then, the HLA*0101 was selected as an allele and finally, the request was submitted. […]

Pipeline specifications

Software tools EpiJen, BCPREDS
Application Immune system analysis
Organisms Escherichia coli, Escherichia coli BL21(DE3)
Diseases Diphtheria
Chemicals Amino Acids