Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 40
Release date: Jun 4 2012
Last update date: Apr 5 2013
Access: Public
Diseases: Hypothalamic Neoplasms, Pituitary Diseases
Chemicals: Hydrocortisone
Dataset link Effect of nonylphenol on gene expression in Atlantic salmon liver, gill, hypothalamus, pituitary, and olfactory rosettes

Experimental Protocol


Microarray analyses were used to compare expression in nonylphenol-injected fish with expression in vehicle-injected fish: eight arrays each for liver, gill, olfactory rosettes, hypothalamus, and pituitary tissues. Total RNA was isolated from the tissues of eight nonylphenol-injected fish (six males and two females) and eight vehicle-injected fish (two males and six females) and reverse transcribed separately (not pooled); each slide represents a biological replicate. For each tissue, the eight arrays were balanced for dye: nonylphenol-injected fish were labeled with Alexa Fluor 555 and vehicle-injected fish were labeled with Alexa Fluor 647 on four slides, nonylphenol-injected fish were labeled with Alexa Fluor 647 and vehicle-injected fish were labeled with Alexa Fluor 555 on four slides. Liver, gill, hypothalamus, pituitary, and olfactory rosette tissues were analyzed separately.

Repositories


GEO

GSE33715

ArrayExpress

E-GEOD-33715

BioProject

PRJNA148479

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Contact


Laura Robertson