Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 10
Release date: Dec 1 2010
Last update date: Apr 6 2016
Access: Public
Chemicals: Glucose
Computational protocol: Comet, DTASelect, UniProtKB
Dataset link Transcriptome analysis of glucose starved Bacillus subtilis cells

Experimental Protocol


Cells were grown in a minimal medium with glucose and L-malate (0.05 % each) to induce glucose starvation after growth to an OD500 of 1.0. Samples were taken at different time points along the growth curve (exponential growth, transition phase, transition phase +30, +60 and +120 minutes). Microarray hybridizations were performed with RNA from two biological replicates. The individual samples were labeled with Cy5; a reference pool containing equal amounts of RNA from all 10 samples was labeled with Cy3.

Repositories


GEO

GSE24058

ArrayExpress

E-GEOD-24058

BioProject

PRJNA130141

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Contact


Ulrike Mäder
Ulrike Mäder

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