Dataset features

Specifications


Dataset type: Expression profiling by RT-PCR, Transcriptome or Gene expression
Number of samples: 16
Release date: Dec 1 2014
Last update date: Jan 8 2018
Access: Public
Dataset link miRNA profiling of Macaca mulatta intact colon samples during chronic SIV infection

Experimental Protocol


We performed TaqMan Low Density Array based high throughput miRNA analysis on intact colon tissue from 12 chronically SIV-infected and 4 uninfected control macaques. All SIV-infected animals were inoculated intravenously with 100TCID50 of SIV. Out of the ten, one animal each was at 7, 8 and 10DPI (days post infection), 3 each at 13 and 21DPI, and 1 at 29DPI. microRNA reverse transcription and preamplification was performed according to the manufacturer’s recommendation. Data analysis was performed using RQ Manager 1.2.2 and DataAssist v3.01 software. Data was normalized using Global normalization method and multiple comparisons correction was performed using Benjamini-Hochberg method.

Repositories


GEO

GSE60773

BioProject

PRJNA259527

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Contact


Mahesh Mohan