|Number of samples:||23|
|Release date:||May 3 2018|
|Last update date:||Nov 28 2018|
|Diseases:||Breast Neoplasms, Neoplasms|
|Dataset link||mRNA-seq Whole Transcriptome Profiling of Fresh Frozen versus Archived Fixed Tissues|
We perform an unbiased evaluation of RNA-seq of archived tumor tissues by comparing the same library preparation methods for both FF and FFPE matched tumor samples and for small amounts of total RNA starting material. We have 3 matched FF/FFPE tumor samples with a moderate archival time of about 4-5 years (T1=T3), and additional 3 FFPE tumor samples archived for more than 10 years (T4-T6). all samples were tested with two protocols: illumina Truseq RNA after poly(A) selection (mRNA-seq); and Truseq after ribosomal depletion (RiboZero). Several initial amounts of starting material was tested for eacg protocol.