Dataset features


Application: Gene expression microarray analysis
Number of samples: 60
Release date: Oct 30 2008
Last update date: May 15 2017
Access: Public
Diseases: Glioblastoma, Neoplasms
Chemicals: Etoposide
Dataset link Integrative Genome-wide Analysis of Glioblastoma.

Experimental Protocol

Glioblastoma multiforme (n=19) and non-neoplastic brain samples (n=4) were analysed. CGH-array were performed in a dye-swap experiment. Tumor DNA was hybridized with the corresponding patient blood DNA as reference to identify somatic changes only. Whole genome expression microarray were performed for the same GBMs with particular care to provide paired data sets. For each brain tumor sample: a snap-frozen tumor block was cryodissected in 10µm sections. The first section as well as sections obtained every 100µm were stained to analyze tissue with at least 70 percents of tumoral cells and to exclude necrotic areas and widespread blood vessels. The adjacent sections were alternatively pooled in different tubes for RNA and DNA extractions, thus allowing a comparison between closely related biological materials. The non-neoplastic brain samples (N) were also analysed by expression microarray to provide gene expression measurements expressed as log2 ratio (GBM vs mean N) and to allow testing procedure.








Marie de Tayrac