Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 8
Release date: Mar 8 2018
Last update date: Sep 21 2018
Access: Public
Taxon: Drosophila melanogaster
Dataset link Neural Lineage Progression Controlled by a Temporal Proliferation Program

Experimental Protocol


We generated two quadruple transgenic UAS combinations: UAS-ase, -SoxN, -wor, -Kr (denoted UAS-Quad), and dominant-negative or -activated versions of three of the same of genes, UAS-ase-Vp16, -SoxN-EnR, -wor-EnR, with UAS-Kr encoding a wild type protein (denoted UAS-QuadD). UAS-Quad and UAS-QuadD misexpression were driven from da-Gal4. To analyze the transcriptomic effects of the Quad and QuadD misexpression, we conducted RNAseq analysis for two separate set of experiments: 1) da-Gal4/+ (control) VS da-Gal4/UAS-Quad), and 2) da-Gal4/+ (control) VS da-Gall4/UAS-QuadD. Within each experiment, we generated two biological replicates per genotype (control and gain-of-function). In total, we thus analyzed 8 samples (4 per each experiment; two replicates for control and two replicates for gain-of-function).

Repositories


GEO

GSE103660

ENA

SRP117179

BioProject

PRJNA403855

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Contact


shahrzad Bahrampour