|Application:||SNP array data analysis, aCGH data analysis|
|Number of samples:||24|
|Release date:||Mar 18 2009|
|Last update date:||Jan 18 2013|
|Diseases:||Adenocarcinoma, Carcinoma, Squamous Cell|
|Dataset link||Distinct DNA methylation profiles between adenocarcinoma and squamous cell carcinoma of human uterine cervix|
In this project, we investigated the promoter methylation status in human cervical adenocarcinoma (ACA) and squamous cell carcinoma (SCC) to identify genes with tumor type-specific promoter DNA methylation patterns. Six ACA and four SCC tissue specimens from bulky tumor masses (clinical stage Ib2- IIb) were selected to minimize contamination from normal tissues. Another set of hybridizations was performed using an in situ synthesized high-resolution oligonucleotide microarray (NimbleGen Inc), to increase the reliability of the data. This chip was designed to cover the same promoters as the in-house microarray. Four pools of samples were used to reduce the number of arrays used. These pools consisted of two sets of three ACA samples (total of six ACA samples) and two sets of two SCC samples (total of four SCC samples).