|Application:||Gene expression microarray analysis|
|Number of samples:||24|
|Release date:||Jan 1 2011|
|Last update date:||Mar 21 2012|
|Dataset link||Functional genomic analysis of a nitrate reductase knockdown relative to wild-type in model-ecosystems (mesocosm)|
Design: Expression profiles were generated for each monoculture population by pooling 100 leaves per mesocosm into one sample. This resulted in 6 biological replicates for each genotype per generation. Thus, a total of 48 samples were generated (6 wild-type + 6 nia2 mutants x 2 generations x 2 CO2 treatments) and hybridized onto microarrays. We used a direct loop design analyzing generations separately (diagrams of hybridization designs are linked as supplementary PDF files).
David J Weston