Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 24
Release date: Jan 1 2011
Last update date: Mar 21 2012
Access: Public
Chemicals: Nitrates
Dataset link Functional genomic analysis of a nitrate reductase knockdown relative to wild-type in model-ecosystems (mesocosm)

Experimental Protocol


Design: Expression profiles were generated for each monoculture population by pooling 100 leaves per mesocosm into one sample. This resulted in 6 biological replicates for each genotype per generation. Thus, a total of 48 samples were generated (6 wild-type + 6 nia2 mutants x 2 generations x 2 CO2 treatments) and hybridized onto microarrays. We used a direct loop design analyzing generations separately (diagrams of hybridization designs are linked as supplementary PDF files).

Repositories


GEO

GSE16068

ArrayExpress

E-GEOD-16068

BioProject

PRJNA115491

Download


Contact


David Weston
David J Weston