Dataset features

Specifications


Dataset type: Expression profiling by high throughput sequencing, Genome binding/occupancy profiling by high throughput sequencing
Number of samples: 31
Release date: Jan 14 2019
Last update date: Feb 14 2019
Access: Public
Dataset link Xist RNA antagonizes the chromatin remodeler, BRG1, from the inactive X chromosome

Experimental Protocol


The role of Xist RNA as a safeguard of the Xi chromsome during X-chromosome inactivation (XCI) was explored by performing DNA accessibility assay (ATAC-seq) in WT and Xist-KO fibroblast cells. Xist's role in repulsion of the catalytic subunit of SWI/SNF complex, BRG1, was determined by native chromatin immunoprecipitation (ChIP-seq) in these cells. Xist's role on gene regulation during XCI maintenance was investigated by genome-wide expression profiling (RNA-seq).

Repositories


GEO

GSE109395

ENA

SRP131076

BioProject

PRJNA430728

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