Dataset features


Application: Gene expression microarray analysis
Number of samples: 8
Release date: Aug 18 2006
Last update date: Mar 16 2012
Access: Public
Dataset link Evaluation of the performance of the Arabidopsis Pathoarray 464_001: an example of detailed analysis of mutants

Experimental Protocol

This experiment consists of two biological replicates of four samples (Col-0, rps2-101C, ndr1-1 and ndr1-1blind. The ndr1-1blind was included as a fourth genotype whose identity was unknown to the experimenter and identified as ndr1-1 by genotyping.) The profiles of the two ndr1-1 were used to set false discovery rate empirically in significant analysis of microarray (Tusher et al., 2001). A more conservative FDR than the FDR determined empirically using two ndr1-1 was used to analyze other pairwise comparisons. For each genotype, two leaves per plant were pooled from three pots to prepare total RNA.