Computational protocol: Elucidation of Motifs in Ribosomal Protein S9 That Mediate Its Nucleolar Localization and Binding to NPM1/Nucleophosmin

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Protocol publication

[…] NoD, nucleolar localization sequence detector, provides predictions of nucleolar localization sequences in eukaryotic proteins (http://www.compbio.dundee.ac.uk/nod). Nuclear localization signals were predicted using PSORT II (http://www.psort.org/). NetNES 1.1 predicts leucine-rich nuclear export signals (NES) in eukaryotic proteins (http://www.cbs.dtu.dk/services/NetNES/). Multiple sequence alignments were made with ClustalW (http://www.ebi.ac.uk/Tools/msa/clustalw2/). […]

Pipeline specifications

Software tools PSORT, NetNES, Clustal W
Application Protein sequence analysis
Organisms Homo sapiens
Chemicals Silver