|Application:||Gene expression microarray analysis|
|Number of samples:||17|
|Release date:||Feb 20 2015|
|Last update date:||Feb 21 2018|
|Diseases:||Alzheimer Disease, Deficiency Diseases, Tooth Loss|
|Dataset link||In vivo response of microglia to Aβ and prostaglandin-E2 EP2 receptor signaling|
8 month-old C57BL/6 mice, of the genotype CD11b-Cre; EP2+/+ or CD11b-Cre; EP2lox/lox, were injected I.C.V. with either Aβ or vehicle. 48 hours after injection, the mice were sacrificed and transcardially perfused with cold heparinized 0.9% NaCl. Brains were then removed from the mice and pooled, two brains of the same genotype per sample, to ensure adequate cell and RNA yield. The brains were then enzymatically dissociated for microglia isolation using the Neural Tissue Dissociation Kit (P), MACS Separation Columns (LS), and magnetic CD11b Microbeads from Miltenyi Biotec according to the manufacturer's protocol. Immediately after isolating the microglia, RNA was extracted from the cells for microarray analysis.
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