Dataset features

Specifications


Application: BS-seq analysis
Number of samples: 18
Release date: Dec 31 2017
Last update date: Nov 30 2018
Access: Public
Diseases: Deficiency Diseases
Dataset link B cell differentiation is limited by de novo DNA methylation [RRBS]

Experimental Protocol


Naïve lymph node B cells (B220+ GL7- Fas-), Phycoerythrin-specific germinal center B cells (B220+ GL7+ Fas+ PE+), and bone marrow plasma cells (CD138+) were compared between Cd19cre/wtDnmt3afl/flDnmt3bfl/fl (Dnmt3-deficient) and littermate control Cd19wt/wtDnmt3afl/flDnmt3bfl/fl (Dnmt3-sufficient) mice using RRBS, RNA-seq, and ATAC-seq. Naïve lymph node B cells were taken from naïve mice, whereas PE-specific germinal center B cells and bone marrow plasma cells were isolated from mice that had been immunized with phycoerythrin 30 days prior. This Series includes the RRBS component of the study.

Repositories


GEO

GSE89467

ENA

SRP092487

BioProject

PRJNA352268

Download


Contact


Benjamin Barwick

Dataset Statistics

info

Citations per year

Dataset publication