Computational protocol: Cucumarioside A2-2 Causes Macrophage Activation in Mouse Spleen

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Protocol publication

[…] Spleen paraffin-embedded sections (7 μm) were stained with H&E according to the standard procedure for morphological tissue analysis. All preparations were examined by light microscopy (Axio Image Z2, Carl Zeiss, Oberkochen, Germany), images were captured using a digital camera (AxioCam HRc, Carl Zeiss, Oberkochen, Germany) and stored as TIFF files. The resulting images were processed and analyzed using ImageJ software (NIH, Rockville, MD, USA). The area of iba-1-, iNOS-, IL1β-positive macrophages in the white and red pulp spleen immunohistochemical staining was determined using ImageJ software (NIH, Rockville, MD, USA) (plugin: IHC Toolbox). The number of images for counting was at least 60 per group. […]

Pipeline specifications

Software tools ImageJ, IHC Toolbox
Application Microscopic phenotype analysis
Organisms Mus musculus, Cucumis sativus