Dataset features

Specifications


Application: Ribo-seq analysis
Number of samples: 28
Release date: Nov 12 2011
Last update date: May 10 2018
Access: Public
Chemicals: Sirolimus
Dataset link Initiation Pausing of 80S Ribosome on mRNA Is Governed by 5’UTR and Responsive to mTORC1 Signaling (Microarray)

Experimental Protocol


Untreated TSC2 WT MEFs, TSC2 KO MEFs and TSC2 WT MEFs, TSC2 KO MEFs treated with 20nM rapamycin for 30 minutes or 3hours were harvested for ribosme profiling. The fraction samples were pooled into three groups based on velocity sedimentation: single ribosome fraction (Small group), fractions with 2 ~ 4 ribosomes (Medium group), and the one with ≥5 ribosomes (Large group). RNA were extracted from the whole cell lysis and each fraction group.

Repositories


GEO

GSE25331

ArrayExpress

E-GEOD-25331

BioProject

PRJNA142553

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Contact


Shu-Bing Qian