Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 26
Release date: Mar 1 2010
Last update date: Mar 20 2012
Access: Public
Diseases: Gastrointestinal Diseases
Dataset link Temporal development of necrotizing enterocolitis in the preterm pig

Experimental Protocol


Pigs from treatment groups TPN (n=5), and 8 h (n=5) and 24 h (n=5-6) FORM and COLOS were randomly selected for microarray analysis. Equal amounts of total distal small intestinal RNA from all pigs was pooled to make the reference sample. Samples and reference pool were labelled with Oyster 550 and 650, respectively. The in-house spotted porcine oligonucleotide microarray version 4 (POM4) is a low density microarray consisting of 384 different oligonucleotide probes representing more than 200 different immune related genes and eight different array control oligonucleotides (ArrayControl; Ambion, Nærum, Denmark). The immunologically relevant 60-70mer oligonucleotide probes represent interferons and interleukins (and receptors), chemokines (and receptors), acute phase proteins, apoptosis-related factors and sequences with relevance to Toll-like receptors and their intracellular signalling pathways.

Repositories


GEO

GSE13516

ArrayExpress

E-GEOD-13516

BioProject

PRJNA110123

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Contact


Jayda Siggers