Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 23
Release date: Nov 9 2011
Last update date: Feb 22 2018
Access: Public
Chemicals: Benzo(a)pyrene, Dibutyl Phthalate, Guaiacol, Carbaryl
Dataset link FTC-238/hrTPO/RSK008 treated with thyroid peroxidase-disrupting chemicals

Experimental Protocol


FTC-238/hrTPO/RSK008 cells were seeded and after incubation for 24 h at 37C, the cells were treated with non-toxic dose for 48 h. And after total RNA isolation, gene expression analysis was conducted using a 8x60-k or 4x44-k whole human genome microarray. Labeling and hybridization were performed using a FairPlay microarray labeling kit, followed by the coupling of Cy3 (controls) or Cy5 (treated samples) dye. The hybridized slides were scanned using a GenePix 4000B microarray scanner, and the images were analyzed using GenePix 4.1 software to obtain gene expression ratios. The fluorescence intensity of each spot was calculated by local median background subtraction. We then used the robust scatter-plot smoother LOWESS function to perform intensity-dependent normalization of gene expression. Scatter-plot analysis was performed using Microsoft Excel 2000. A significance analysis of microarray (SAM) was performed for genes with significant changes in expression.

Repositories


GEO

GSE30961

ArrayExpress

E-GEOD-30961

BioProject

PRJNA146159

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mee song