Computational protocol: Peptide specific monoclonal antibodies of Leptospiral LigA for acute diagnosis of leptospirosis

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Protocol publication

[…] The protein sequence of LK90 with accession numbers AAB53736 was retrieved from GenBank and subjected for B-cell epitope prediction using BCPred. B-cell epitopes having BCPred score >0.9 and VaxiJEN score >0.4 were selected for further study. [...] Data were analyzed using Statistica software package version 6.0 (StatSoft, Tulsa, OK). Cut-off values were defined as the corresponding Mean + 2 SD from the sera of healthy controls. Sensitivity was defined as the percentage of the laboratory-confirmed cases of leptospirosis whose serum samples gave mean ODs greater than the relevant cutoff value. Specificity was calculated as the percentage of the control individuals whose samples gave mean ODs below the relevant cutoff value. The positive and negative predictive values (PPV and NPV, respectively) are the proportions of true positives and true negatives, respectively. The percent agreement between the results of the recombinant protein-based ELISA and the results of the MAT, and the corresponding kappa coefficients, were determined using Epi Info version 6.0 (Centers for Disease Control and Prevention, Atlanta, GA). Graphs were made using SigmaPlot version 11.0 (Systat Software, Inc.) and GraphPad Prism version 5.0 (GraphPad, San Diego, CA). ed. […]

Pipeline specifications

Software tools VaxiJen, Statistica, SigmaPlot
Applications Immune system analysis, Miscellaneous
Organisms Homo sapiens
Diseases Leptospirosis
Chemicals Amino Acids