|Application:||Gene expression microarray analysis|
|Number of samples:||2|
|Release date:||Nov 25 2009|
|Last update date:||Jan 12 2017|
|Dataset link||Interferon-γ-responsive non-hematopoietic cells regulate the immune response to Mycobacterium tuberculosis|
Bone marrow chimera were created by γ-irradiation (10 Gy) of recipient mice, Ifngr+/+ (W) or Ifngr-/- (K), and reconstitution by i.v. injection of Ifngr+/+ (W) bone marrow cells. After 6 weeks, the two groups of chimera W→W and W→K were infected via the aerosol route with 100 colony forming units of Mycobacterium tuberculosis strain H37Rv. At 63 days post-infection, 4 W→W mice and 4 W→K mice were euthanized, their lung left lobe removed and processed for total RNA isolation and microarray. The 4 samples from the W→W mice were pooled together and used as control sample whereas the 4 samples from the W→K mice were pooled together and used as experimental sample. The same harvest and sample processing was conducted on day 97 after infection, using 5 W→W mice and 6 W→K mice.