Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 30
Release date: Dec 4 2009
Last update date: Jul 6 2016
Access: Public
Chemicals: Glycerol
Computational protocol: Partek Genomics Suite, GC–RMA, SAM, WormBase, GEO
Dataset link Expression data from wild type C. elegans and 5 osmotic stress resistant mutants exposed to hyper/isotonic environments

Experimental Protocol


Young adult C. elegans were exposed to hypertonic growth plates for varying times prior to RNA extraction and hybridization on Affymetrix microarrays. Since we wished to separate direct response from secondary responses to osmotic stress, we collected worms following short term exposures to hypertonic conditions (15 minutes and 1 hour) and after long term exposure to hypertonic conditions (6 hours or a full generation of growth under hypertonic conditions). We also collected young adults from the osr mutants osm-7, osm-8, osm-11, dpy-9, and dpy-10 for microarray analysis. These mutants were grown under isotonic conditions to determine whether that constitutively activate genes normally regulated by hypertonic stress in wild type animals.

Repositories


GEO

GSE19310

ArrayExpress

E-GEOD-19310

BioProject

PRJNA120905

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Contact


Todd Lamitina

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