Dataset features
Specifications
Application: | Gene expression microarray analysis |
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Number of samples: | 30 |
Release date: | Dec 4 2009 |
Last update date: | Jul 6 2016 |
Access: | Public |
Chemicals: | Glycerol |
Computational protocol: | Partek Genomics Suite, GC–RMA, SAM, WormBase, GEO |
Dataset link | Expression data from wild type C. elegans and 5 osmotic stress resistant mutants exposed to hyper/isotonic environments |
Experimental Protocol
Young adult C. elegans were exposed to hypertonic growth plates for varying times prior to RNA extraction and hybridization on Affymetrix microarrays. Since we wished to separate direct response from secondary responses to osmotic stress, we collected worms following short term exposures to hypertonic conditions (15 minutes and 1 hour) and after long term exposure to hypertonic conditions (6 hours or a full generation of growth under hypertonic conditions). We also collected young adults from the osr mutants osm-7, osm-8, osm-11, dpy-9, and dpy-10 for microarray analysis. These mutants were grown under isotonic conditions to determine whether that constitutively activate genes normally regulated by hypertonic stress in wild type animals.
Repositories
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Contact
Todd Lamitina
Dataset Statistics
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Citations per year
Dataset publication
2010
PLoS One
PMCID: 2814864
PMID: 20126308
DOI: 10.1371/journal.pone.0009010
call_split See protocol