Computational protocol: Structural and physiological analyses in Salsoleae (Chenopodiaceae) indicate multiple transitions among C3, intermediate, and C4 photosynthesis

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Protocol publication

[…] Samples of S. webbii and S. genistoides were added to previously published data sets (; ) for the nuclear ribosomal DNA internal transcribed spacer region (ITS); samples utilized in the analysis are listed in Supplementary Appendix S1 at JXB online. The sequences were aligned using MUSCLE (). The aligned matrix of 110 samples and 724 aligned bases was analysed using RAxML () with the GTR gamma model. Nine species of tribe Caroxyloneae were used as the outgroup based on previous studies (). […]

Pipeline specifications

Software tools MUSCLE, RAxML
Applications Phylogenetics, Nucleotide sequence alignment
Diseases Composite Lymphoma