Dataset features


Application: Gene expression microarray analysis
Number of samples: 17
Release date: Feb 28 2011
Last update date: Aug 23 2018
Access: Public
Diseases: Glucose Metabolism Disorders
Chemicals: Glucose, Streptozocin
Dataset link Gene expression differences in mouse islets after isolation at different time points (0-48hr)

Experimental Protocol

Six independent batches of islets were isolated. For each batch, islets from 4 C57BL/6 mice were pooled, and were divided into 3 groups, which were harvested at 0 h, 24 h and 48 h after culture. Total RNA was extracted from the islets with QIAGEN RNeasy Micro Kits. After reverse transcription, cDNA served as a template for PCR-based cRNA amplification. It was then reverse-transcribed to produce second-generation cDNA, which was employed to generate biotin-labelled cRNA probes. The probes were hybridized on GeneChip Mouse Genome 430 2.0 Array (Affymetrix, Santa Clara, CA), which contains 39,000 transcripts. DNA microarray analysis was performed at the McGill University Genome Quebec Innovation Centre. For each time point, 6 chips were hybridized with cRNA from the 6 batches of isolated islets. For samples collected at 24 and 48 hours (6 batches for each time point), if the mean signal strength of a gene had above 4-fold difference over that of the 0 h samples, it was selected for reverse polymerase chain reaction (PCR) confirmation. Details of the experimental protocols and data analysis procedure can be found in . Batch 1: YF050524MOT01, YF050524MOT07, YF050524MOT13 Batch 2: YF050524MOT02, YF050524MOT08, YF050524MOT14 Batch 3: YF050524MOT03, YF050524MOT09, YF050524MOT15 Batch 4: YF050524MOT04, YF050524MOT10, YF050524MOT16 Batch 5: YF050524MOT11, YF050524MOT17 Batch 6: YF050524MOT06, YF050524MOT12, YF050524MOT18










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