Dataset features

Specifications


Dataset type: Expression profiling by array
Number of samples: 15
Release date: Feb 6 2019
Last update date: Feb 6 2019
Access: Public
Dataset link Transcriptional profiling of intestinal epithelial cells in response to Enterococcus gallinarum MRx0518

Experimental Protocol


Confluent monolayers of HT29-MTX cells (ten days post seeding) were incubated with treatments of live MRx0518 and heat-killed MRx0518 at a multiplicity of infection (MOI) of 100 bacterial cells to 1 human cell, and an equivalent dilution of MRx0518 cell-free culture supernatant for 3 h at 37 °C in anaerobic conditions. Untreated cells and cells treated with bacterial culture medium (YCFA) were used as assay controls. Following incubation, RNA was isolated and hybridised to Affymetrix Human Transcriptome Array 2.0 GeneChips (GEO Accession Number GPL17586). Three independent biological replicates of each condition were carried out.

Repositories


GEO

GSE122232

BioProject

PRJNA504039

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