|Number of samples:||18|
|Release date:||May 8 2018|
|Last update date:||Nov 29 2018|
|Computational protocol:||Bowtie, MACS, SeqPlots|
|Dataset link||Different Chromatin and DNA Sequence Characteristics Define Glucocorticoid Receptor Binding Sites that are Blocked or Not Blocked by Coregulator Hic-5 [ChIP-seq]|
Since Hic-5 inhibits the actions of CHD9 and BRM, we performed double depletion of Hic-5 and CHD9 or Hic-5 and BRM in U2OS-GR⍺ cell using siRNA and examined GR occupancy at 1 hour dexamethasone treatment. Single depletion of CHD9, BRM, Hic-5 and control siRNA (siNS) followed by hormone treatment were performed as contrast and control.
Citations per year
DOI: 10.1371/journal.pone.0196965call_split See protocol