Dataset features

Specifications


Application: ChIP-seq analysis
Number of samples: 18
Release date: May 8 2018
Last update date: Nov 29 2018
Access: Public
Genes: TGFB1I1, CHD9
Computational protocol: Bowtie, MACS, SeqPlots
Dataset link Different Chromatin and DNA Sequence Characteristics Define Glucocorticoid Receptor Binding Sites that are Blocked or Not Blocked by Coregulator Hic-5 [ChIP-seq]

Experimental Protocol


Since Hic-5 inhibits the actions of CHD9 and BRM, we performed double depletion of Hic-5 and CHD9 or Hic-5 and BRM in U2OS-GR⍺ cell using siRNA and examined GR occupancy at 1 hour dexamethasone treatment. Single depletion of CHD9, BRM, Hic-5 and control siRNA (siNS) followed by hormone treatment were performed as contrast and control.

Repositories


GEO

GSE109383

ENA

SRP131064

BioProject

PRJNA430719

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Contact


Brian Lee

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