Computational protocol: Histone Reader BRWD1 Targets and Restricts Recombination to the Igk Locus

Similar protocols

Protocol publication

[…] All raw sequence data was quality trimmed to a minimum phred score of 20 using trimmomatic. Alignment to reference genome mm9 was done with BWA. For ATAC-Seq data, read pairs where one pair passed quality trimming but the other did not were aligned separately and merged with the paired-end alignments. PCR duplicates were removed using Picard MarkDuplicates and alignments with an edit distance greater than 2 to the reference, or those were mapped multiple times to the reference, were removed. […]

Pipeline specifications

Software tools Trimmomatic, BWA, Picard
Application Nucleotide sequence alignment