Computational protocol: Draft Genome Sequence of Lactobacillus delbrueckii subsp. bulgaricus CRL871, a Folate-Producing Strain Isolated from a Northwestern Argentinian Yogurt

Similar protocols

Protocol publication

[…] Lactobacillus delbrueckii subsp. bulgaricus is a lactic acid bacterium (LAB) that is widely used as a starter culture in the dairy industry for the manufacture of fermented milk products, such as yogurt, when combined with Streptococcus thermophilus. Historically, L. delbrueckii subsp. bulgaricus was described as a folate consumer that normally took up folates produced by S. thermophilus. L. delbrueckii subsp. bulgaricus CRL871, isolated from an artisanal yogurt in the northwestern region of Argentina, showed the ability to produce folate when grown in folate-free culture medium () and also in milk (). The ability to produce folate confers to this strain an important biotechnological ability, allowing the development of novel fermented milk products naturally bioenriched in folates, representing a viable, safe, and affordable biotechnological alternative to folic acid fortification. Here, we report the draft genome sequence of the first folate-producing L. delbrueckii subsp. bulgaricus strain described. The genome sequence of this strain allowed the identification of genes responsible for folate-producing enzymes and its future biotechnological application in food industry.The genome of L. delbrueckii subsp. bulgaricus CRL871 was sequenced using a whole-genome shotgun (WGS) strategy (40-fold genome coverage, N50 contig length of 59,563 bp) with an Ion Torrent personal genome machine (Life Technologies). Quality filtered reads were assembled using the Ngen (DNASTAR) assembler, giving 63 contigs that are more than 2,000 bp in length. Genome annotation was done using the NCBI Prokaryotic Genome Annotation Pipeline (http://www.ncbi.nlm.nih.gov/genome/annotation_prok/) and from the Rapid Anotations using Subsystems Technology (RAST) server (). tRNAs and rRNAs were identified by tRNAscan-SE and RNAmmer, respectively (, ).The genome size consists of 2,063,981 bp with a mean G+C content of 49.1%. A total of 2,313 coding sequences (CDS), 1,932 genes with predicted function, 122 structural tRNAs, and 39 rRNA were predicted. Additionally, there are 264 RAST subsystems represented in the chromosome, which represent only 47% of the assigned sequences.As was previously mentioned, L. delbrueckii subsp. bulgaricus CRL871 showed the ability to synthetize folates when grow in a folate-free culture medium () and in milk (). Genomes from different strains of L. bulgaricus showed sequences coding enzymes responsible for folates synthesis. However, none of these strains have been described as folate producers. In silico genomic analysis of L. delbrueckii subsp. bulgaricus CRL871 revealed the presence of folA, folC, folP, and phoA genes, which are responsible for de novo synthesis of folates. The presence of these genes together with the ability to synthetize folate gives this strain a very important biotechnological potential in the dairy industry for the development of yogurt naturally bio-enriched in folate, as was recently reported (, ). […]

Pipeline specifications

Software tools PGAP, RAST, tRNAscan-SE, RNAmmer
Applications Genome annotation, WGS analysis
Organisms Lactobacillus delbrueckii subsp. bulgaricus
Chemicals Folic Acid